ISSN 2394-5125
 

Research Article 


DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF APIGENIN AND LUTEOLIN IN COMMERCIAL FORMULATION

S. Gomathy, S.T. Narenderan, S.N. Meyyanathan, B.Gowramma.

Abstract
The goal of the study is to establish a simple and precise HPLC method from its commercial formulation for the quantification of Apigenin and Luteolin. The separation of the analytes was performed by Hibar Lichrospher C8 (150 x 4.6mm i.d., 5μ) column with the mobile phase consisting of methanol and 0.5 % trifluroacetic acid (80:20 %, v/v) at 1 ml/min flow rate and 269 nm wavelength detection. The method developed was found to be linear over the concentration ranging from 10 to 110 ng/ml with > 0.99 correlation coefficient (R2) for both Apigenin and Luteolin. The results obtained from the validation studies states that the method developed was found to be simple, precise and accurate in accordance with the ICH guidelines. Through this original research work attempt was therefore made to standardize selected marketed formulations using apigenin and luteolin as an active biological marker for simultaneous quantification through established and validated HPLC techniques.

Key words: Apigenin; Luteolin; HPLC; ICH guidelines; commercial formulations.


 
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How to Cite this Article
Pubmed Style

S. Gomathy, S.T. Narenderan, S.N. Meyyanathan, B.Gowramma. DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF APIGENIN AND LUTEOLIN IN COMMERCIAL FORMULATION. JCR. 2020; 7(19): 4785-4790. doi:10.31838/jcr.07.19.560


Web Style

S. Gomathy, S.T. Narenderan, S.N. Meyyanathan, B.Gowramma. DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF APIGENIN AND LUTEOLIN IN COMMERCIAL FORMULATION. http://www.jcreview.com/?mno=127847 [Access: September 14, 2020]. doi:10.31838/jcr.07.19.560


AMA (American Medical Association) Style

S. Gomathy, S.T. Narenderan, S.N. Meyyanathan, B.Gowramma. DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF APIGENIN AND LUTEOLIN IN COMMERCIAL FORMULATION. JCR. 2020; 7(19): 4785-4790. doi:10.31838/jcr.07.19.560



Vancouver/ICMJE Style

S. Gomathy, S.T. Narenderan, S.N. Meyyanathan, B.Gowramma. DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF APIGENIN AND LUTEOLIN IN COMMERCIAL FORMULATION. JCR. (2020), [cited September 14, 2020]; 7(19): 4785-4790. doi:10.31838/jcr.07.19.560



Harvard Style

S. Gomathy, S.T. Narenderan, S.N. Meyyanathan, B.Gowramma (2020) DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF APIGENIN AND LUTEOLIN IN COMMERCIAL FORMULATION. JCR, 7 (19), 4785-4790. doi:10.31838/jcr.07.19.560



Turabian Style

S. Gomathy, S.T. Narenderan, S.N. Meyyanathan, B.Gowramma. 2020. DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF APIGENIN AND LUTEOLIN IN COMMERCIAL FORMULATION. Journal of Critical Reviews, 7 (19), 4785-4790. doi:10.31838/jcr.07.19.560



Chicago Style

S. Gomathy, S.T. Narenderan, S.N. Meyyanathan, B.Gowramma. "DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF APIGENIN AND LUTEOLIN IN COMMERCIAL FORMULATION." Journal of Critical Reviews 7 (2020), 4785-4790. doi:10.31838/jcr.07.19.560



MLA (The Modern Language Association) Style

S. Gomathy, S.T. Narenderan, S.N. Meyyanathan, B.Gowramma. "DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF APIGENIN AND LUTEOLIN IN COMMERCIAL FORMULATION." Journal of Critical Reviews 7.19 (2020), 4785-4790. Print. doi:10.31838/jcr.07.19.560



APA (American Psychological Association) Style

S. Gomathy, S.T. Narenderan, S.N. Meyyanathan, B.Gowramma (2020) DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF APIGENIN AND LUTEOLIN IN COMMERCIAL FORMULATION. Journal of Critical Reviews, 7 (19), 4785-4790. doi:10.31838/jcr.07.19.560