ISSN 2394-5125
 

Research Article 


STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION OF METRONIDAZOLE, TETRACYCLINE AND BISMUTHSUBCITRATE BY USING RP-HPLC

Srinivasu Navuluri, Gopikrishna Yenduri.

Abstract
A novel stability indicating method is developed by using High performance liquid chromatography
(HPLC) and evaluated for the simultaneous quantification of Metronidazole(MDZ), Tetracycline(TTC) and
Bismuthsubcitrate(BMSC) in the capsule dosage form. Chromatographic Separation was achieved by using the
instrument Waters made Alliance HPLC system equipped with auto sampler, UV detector and Phenominex C18 (4.6 mm
x 250 mm) 5 μm column with a mobile phase composition of 0.1% TFA (pH 4.5) and the solvent Acetonitrile in the ratio
75:25 v/v delivered at a flow rate of 1.0ml/min and the detection was carried out using UV detector at the wavelength of
281 nm. The three active pharmaceutical ingredients were extracted from tablet dosage form using diluents (Mobile
phase). The retention times for MDZ, TTC and BMSC were 2.287, 2.816 and 5.526 minutes respectively. Calibration
curves obtained linearity over concentration graphs was linear and the proposed method showed very good recoveries for
both bulk and tablet dosage forms. The correlation coefficient values of linearity for all three active ingredients were
found to be 0.999 and the concentration range was 12.5-62.5μg/ml, 12.5-62.5μg/ml and 14-70g/ml respectively. The
results of the study indicated that the proposed RP‐HPLC method is fast, simple, precise, accurate, robust, and
reproducible, which can be useful for the routine estimation of MDZ, TTC and BMSC in pharmaceutical dosage form.
The method developed was very selective for the simultaneous quantification of MDZ, TTC and BMSC, because
effective separation of the drugs from their degradation products. This method can be employed as a stability-indicating
one.

Key words: Metronidazole(MDZ), Tetracycline(TTC), Bismuthsubcitrate(BMSC), RP‐HPLC, Simultaneous estimation.


 
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Pubmed Style

Srinivasu Navuluri, Gopikrishna Yenduri. STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION OF METRONIDAZOLE, TETRACYCLINE AND BISMUTHSUBCITRATE BY USING RP-HPLC . JCR. 2021; 8(1): 776-784. doi:10.31838/jcr.08.01.86


Web Style

Srinivasu Navuluri, Gopikrishna Yenduri. STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION OF METRONIDAZOLE, TETRACYCLINE AND BISMUTHSUBCITRATE BY USING RP-HPLC . http://www.jcreview.com/?mno=58656 [Access: April 17, 2021]. doi:10.31838/jcr.08.01.86


AMA (American Medical Association) Style

Srinivasu Navuluri, Gopikrishna Yenduri. STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION OF METRONIDAZOLE, TETRACYCLINE AND BISMUTHSUBCITRATE BY USING RP-HPLC . JCR. 2021; 8(1): 776-784. doi:10.31838/jcr.08.01.86



Vancouver/ICMJE Style

Srinivasu Navuluri, Gopikrishna Yenduri. STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION OF METRONIDAZOLE, TETRACYCLINE AND BISMUTHSUBCITRATE BY USING RP-HPLC . JCR. (2021), [cited April 17, 2021]; 8(1): 776-784. doi:10.31838/jcr.08.01.86



Harvard Style

Srinivasu Navuluri, Gopikrishna Yenduri (2021) STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION OF METRONIDAZOLE, TETRACYCLINE AND BISMUTHSUBCITRATE BY USING RP-HPLC . JCR, 8 (1), 776-784. doi:10.31838/jcr.08.01.86



Turabian Style

Srinivasu Navuluri, Gopikrishna Yenduri. 2021. STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION OF METRONIDAZOLE, TETRACYCLINE AND BISMUTHSUBCITRATE BY USING RP-HPLC . Journal of Critical Reviews, 8 (1), 776-784. doi:10.31838/jcr.08.01.86



Chicago Style

Srinivasu Navuluri, Gopikrishna Yenduri. "STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION OF METRONIDAZOLE, TETRACYCLINE AND BISMUTHSUBCITRATE BY USING RP-HPLC ." Journal of Critical Reviews 8 (2021), 776-784. doi:10.31838/jcr.08.01.86



MLA (The Modern Language Association) Style

Srinivasu Navuluri, Gopikrishna Yenduri. "STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION OF METRONIDAZOLE, TETRACYCLINE AND BISMUTHSUBCITRATE BY USING RP-HPLC ." Journal of Critical Reviews 8.1 (2021), 776-784. Print. doi:10.31838/jcr.08.01.86



APA (American Psychological Association) Style

Srinivasu Navuluri, Gopikrishna Yenduri (2021) STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION OF METRONIDAZOLE, TETRACYCLINE AND BISMUTHSUBCITRATE BY USING RP-HPLC . Journal of Critical Reviews, 8 (1), 776-784. doi:10.31838/jcr.08.01.86