Abstract

In the present study, Aeromonas hydrophila, a bacteria isolated from infected goldfish (Carassius auratus), was tested for its antibacterial efficacy against Tridax procumbens leaf � diethyl ether extract. Diffusion disc plates on agar were used to measure the antibacterial activity. Different concentrations 25 % (0.25 �l), 50 % (0.5 �l), 75 % (0.75 �l) and 100 % (1.0 �l) of T. procumbens leaf - diethyl ether extract was tested against A. hydrophila. The T. procumbens leaf � diethyl ether extract was exhibited a significant level of inhibition against A. hydrophila. Maximum zone of clearance was measured after 24 hours of incubation at 37�C. The result of the present study revealed that the fish pathogen A. hydrophila was exhibited maximum zone of clearence (24 mm) at 100 % concentration of diethyl ether- leaf extract, where as the minimum zone of clearance 10 mm was recorded at 25 % of concentration. Moreover the current analysis was carried out to identify the bioactive constituents. The phytochemical analysis of the T. procumbens leaf �diethyl ether extract showed the presence of tannins, terpenoids, steroids and coumarins. These compounds were exhibited antibacterial activity against the fish pathogen A. hydrophila. As per the result of the present study, it can be concluded that phytochemicals were identified in T. procumbens leaf � diethyl ether extract was effective against A. hydrophila, a microbial pathogen that has infected goldfish (C. auratus).

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