Abstract

Chemical castration is a method of castration by drug. In this way, unlike sterilization of sex glands those are removed through surgery, Glands are undermined by injecting chemicals such as calcium chloride into the tissue. The aim of this study was to assess the effects of calcium chloride solution for injection in rat testis tissue with ultrasonography and histopathology, in analyzing the testis. 20 male Wistar Rats with an average age of 6 months and weighing approximately 250-200 g, clinically healthy, were selected. The mice were then randomly divided into 4 equal groups. The first group was considered as a control group. Then, in the other three groups, after the measurements, 20% calcium chloride was injected into the testicular tissue, so that its tissue was completely filled. Then, on the 7th, 14th and 21st days, samples were taken from the right testicles of the groups for histopathological examination and were examined by hematoxylin and eosin staining. The length, width and echogenicity of all testes on Zero, 7th 14th and 21st days were also examined by ultrasonography. Finally, the results of quantitative and semi-quantitative study of the measured components were statistically analyzed. The results of ultrasonography showed that firstly the injection length and width between the left and right testis there is no significant difference between groups. But on 7th, 14th and 21st days significant difference between them is seen. Secondly, the average length and width of the left and right testicles in both groups during the study (7th, 14th and 21 days after injection) significantly decreased. Thirdly Echogenicity of left and right testis in groups during the experiment significantly reduced. In addition, histopathological examination revealed that thein the testicular tissue of the experimental groups, cellular necrosis, hemorrhage, neutrophilia and calcification was seen. Conclusion: Based on the findings of this study, it can be concluded that chemical sterilization using calcium chloride is an effective method and reduces costs, reduces side effects and eliminates anesthesia. But the effects of these methods appear slowly

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