ISSN 2394-5125
 

Research Article 


Genotypic assessment of UropathogenicEscherichia coli isolated from urinary tract infections

Seyed Amirshayan Makkie, Farhad Safarpoor Dehkordi.

Abstract
UropathogenicEscherichia coli is considered the most important infectious agent responsible for the occurrence of
urinary tract infections. The present survey was conducted to assess the genotypic pattern of virulence factors
amongst the 40 UPEC isolates of UTIs. Forty UPEC strains were isolated from patients with UTIs. Isolates were
confirmed using different biochemical tests. The polymerase chain reaction assessed the genotypic distribution of
virulence factors. Findings showed that fimh (90%), pap (85%), cnf1 (70%), hlyA (65%), afa (60%), iron (57.5%),
and iuc (50%) were the most commonly detected virulence factors, However, tsh (5%), usp (12.5%), ompT (20%),
and irp2 (32.5%) were the less commonly detected virulence factors amongst the UPEC strains. Statistically, a
significant difference was found between the distribution of different virulence factors (P<0.05). Considering the
high distribution of UPEC virulence factors amongst isolated bacteria, proper control of the virulent UPEC strains as
an important cause of UTIs should perform in healthcare units. PCR method was defined as a property assay for
rapid and sensitive detection of virulence factors.

Key words: UropathogenicEscherichia coli, Virulence factors, Urine, Urinary Tract Infections, PCR.


 
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Pubmed Style

Seyed Amirshayan Makkie, Farhad Safarpoor Dehkordi. Genotypic assessment of UropathogenicEscherichia coli isolated from urinary tract infections. JCR. 2021; 8(2): 1256-1261. doi:10.31838/jcr.08.02.128


Web Style

Seyed Amirshayan Makkie, Farhad Safarpoor Dehkordi. Genotypic assessment of UropathogenicEscherichia coli isolated from urinary tract infections. http://www.jcreview.com/?mno=85095 [Access: June 03, 2021]. doi:10.31838/jcr.08.02.128


AMA (American Medical Association) Style

Seyed Amirshayan Makkie, Farhad Safarpoor Dehkordi. Genotypic assessment of UropathogenicEscherichia coli isolated from urinary tract infections. JCR. 2021; 8(2): 1256-1261. doi:10.31838/jcr.08.02.128



Vancouver/ICMJE Style

Seyed Amirshayan Makkie, Farhad Safarpoor Dehkordi. Genotypic assessment of UropathogenicEscherichia coli isolated from urinary tract infections. JCR. (2021), [cited June 03, 2021]; 8(2): 1256-1261. doi:10.31838/jcr.08.02.128



Harvard Style

Seyed Amirshayan Makkie, Farhad Safarpoor Dehkordi (2021) Genotypic assessment of UropathogenicEscherichia coli isolated from urinary tract infections. JCR, 8 (2), 1256-1261. doi:10.31838/jcr.08.02.128



Turabian Style

Seyed Amirshayan Makkie, Farhad Safarpoor Dehkordi. 2021. Genotypic assessment of UropathogenicEscherichia coli isolated from urinary tract infections. Journal of Critical Reviews, 8 (2), 1256-1261. doi:10.31838/jcr.08.02.128



Chicago Style

Seyed Amirshayan Makkie, Farhad Safarpoor Dehkordi. "Genotypic assessment of UropathogenicEscherichia coli isolated from urinary tract infections." Journal of Critical Reviews 8 (2021), 1256-1261. doi:10.31838/jcr.08.02.128



MLA (The Modern Language Association) Style

Seyed Amirshayan Makkie, Farhad Safarpoor Dehkordi. "Genotypic assessment of UropathogenicEscherichia coli isolated from urinary tract infections." Journal of Critical Reviews 8.2 (2021), 1256-1261. Print. doi:10.31838/jcr.08.02.128



APA (American Psychological Association) Style

Seyed Amirshayan Makkie, Farhad Safarpoor Dehkordi (2021) Genotypic assessment of UropathogenicEscherichia coli isolated from urinary tract infections. Journal of Critical Reviews, 8 (2), 1256-1261. doi:10.31838/jcr.08.02.128