Abstract

Metagenomics and directed evolution have changed how we look for new enzymes in harsh settings and how we change current enzymes to make them do what we want. Researchers can make enzymes with more activity, stability, and substrate selectivity using next-generation sequencing, site-directed mutagenesis, fusion protein, surface display, and other molecular biology techniques. These enzymes are used in industry. Many large-scale industrial enzyme processes still need to get around the limits of catalytic process activity. This piece talks about how enzymes are used in industry and how metabolic engineering is being used to make enzymes work better and produce more.

Description