STABILITY INDICATING RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF ACYCLOVIR IN BULK AND PHARMACEUTICAL DOSAGE FORM UNDER STRESS DEGRADATION CONDITIONS (2023)
PAVAN KUMAR KASU, B. SATYNARAYANA JCR. 2023: 1-11
A simple, sensitive, specific, and accurate RP-HPLC approach is used to estimate the of acyclovir in tablet and bulk dosage forms. Mobile phase contained 0.1% OPA, and the chromatogram was performed using the Kromosil C18 (4.1 x 250mm, 5µm). With the flow rate of 1 ml/min, acetonitrile that had been diluted with water in a proportion, 60:40 was injected through the column at 30°C. The chosen optimized wavelength was 254.0 nm. Acyclovir's retention time was discovered to be 2.346 minutes. Acyclovir's %RSD was determined to be 0.9%. %RSD of Acyclovir's Repeatability Precision was discovered as 0.5%. Acyclovir had a 99.99% recovery rate. The result of the % assay for acyclovir was 100.08%. Acyclovir's LOD and LOQ values were 0.32 and 0.98, respectively. Acyclovir's regression equation is y = 32813x + 4220. As a result of shorter retention times and shorter run times, the method was created to be simple, cost-effective, and it may be used for routine Quality Control Tests in Industries.
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